| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Helmick, Madison C (15)
|
MIBBD |
25.07 |
26.14 |
1.07 |
| 2 |
Higgins, Lillian E (15)
|
MIBBD |
25.97 |
27.22 |
1.25 |
| 3 |
Hess, Emily K (15)
|
MILCY |
27.10 |
27.66 |
0.56 |
| 4 |
Thome, Marissa A (17)
|
MIBBD |
25.82 |
27.67 |
1.85 |
| 5 |
Dickhudt, Emmalyse I (16)
|
MIBBD |
26.20 |
28.03 |
1.83 |
| 5 |
Grasso, Estella S (15)
|
MIFLY |
26.90 |
28.03 |
1.13 |
| 7 |
Taylor, Lauren K (16)
|
MIFLY |
26.51 |
28.32 |
1.81 |
| 8 |
Gaggos, Kalliope S (15)
|
MIBBD |
27.18 |
28.79 |
1.61 |
| 9 |
Vondell, Lindsay K (15)
|
MIBBD |
28.11 |
28.90 |
0.79 |
| 10 |
Weissman, Sarah A (15)
|
MIBBD |
28.60 |
29.99 |
1.39 |
| 11 |
Fader, Emma (16)
|
MIAABA |
34.02 |
34.47 |
0.45 |
|
Baldwin, Lina (15)
|
MIBWO |
30.03 |
NS |
- |
|
Lapine, Gwendolynn J (15)
|
MISYS |
26.49 |
NS |
- |